Genetic diversity of Azotobacter strains isolated from various soils of Poland
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Date
2023
Journal Title
Journal ISSN
Volume Title
Publisher
Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy w Puławach
Abstract
Description
Free-living nitrogen-fixing bacteria belonging to the
genus Azotobacter are microorganisms commonly occurring in
soil. The genus Azotobacter includes 8 species, with Azotobacter
chroococcum most commonly inhabiting many soils all over the
world. This study aims to isolate and to identify the Azotobacter
bacteria from various soils of Poland using RFLP analysis of the
bacterial Internal Transcribed Spacer (ITS) region and 16S rRNA
gene sequence analysis. 56 strains were isolated from tested soils,
using the dilution-pour plates method. Restriction analysis of the
bacterial ITS region was used for the characterization and dif-
ferentiation of the isolated strains. It indicated that the studied
isolates were not identical. Among the 56 tested strains, 36 were
characterized by the same genotype in ITS-PCR/RFLP analyzes
as the reference strains A. chroococcum DSM281 and DSM2286.
For molecular identification of the isolates, the 16S rRNA gene
was amplified using 27f and 1492r primers and PCR products
were sequenced. These results indicate that the analyzed isolates
from Polish soils belong to one species, namely Azotobacter
chroococcum.
Keywords
Azotobacter chroococcum, soil, nitrogen fixation, genetic diversity, 16S rRNA gene
Citation
Polish Journal of Agronomy
2023, 52, 136–145