Genetic diversity of Azotobacter strains isolated from various soils of Poland

dc.contributor.authorKozieł, Monika
dc.contributor.authorGałązka, Anna
dc.contributor.authorMartyniuk, Stefan
dc.date.accessioned2024-06-10T13:16:03Z
dc.date.available2024-06-10T13:16:03Z
dc.date.issued2023
dc.descriptionFree-living nitrogen-fixing bacteria belonging to the genus Azotobacter are microorganisms commonly occurring in soil. The genus Azotobacter includes 8 species, with Azotobacter chroococcum most commonly inhabiting many soils all over the world. This study aims to isolate and to identify the Azotobacter bacteria from various soils of Poland using RFLP analysis of the bacterial Internal Transcribed Spacer (ITS) region and 16S rRNA gene sequence analysis. 56 strains were isolated from tested soils, using the dilution-pour plates method. Restriction analysis of the bacterial ITS region was used for the characterization and dif- ferentiation of the isolated strains. It indicated that the studied isolates were not identical. Among the 56 tested strains, 36 were characterized by the same genotype in ITS-PCR/RFLP analyzes as the reference strains A. chroococcum DSM281 and DSM2286. For molecular identification of the isolates, the 16S rRNA gene was amplified using 27f and 1492r primers and PCR products were sequenced. These results indicate that the analyzed isolates from Polish soils belong to one species, namely Azotobacter chroococcum.en-US
dc.formatapplication/pdf
dc.identifier.citationPolish Journal of Agronomy 2023, 52, 136–145
dc.identifier.doi10.26114/pja.iung.523.2023.52.14
dc.identifier.issn2081-2789
dc.identifier.urihttps://bc.iung.pl/handle/123456789/1249
dc.language.isoen
dc.publisherInstytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy w Puławachpl-PL
dc.subjectAzotobacter chroococcumen-US
dc.subjectsoilen-US
dc.subjectnitrogen fixationen-US
dc.subjectgenetic diversityen-US
dc.subject16S rRNA geneen-US
dc.titleGenetic diversity of Azotobacter strains isolated from various soils of Polanden-US
dc.typeinfo:eu-repo/semantics/article
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