Comparative phytochemical, antioxidant, and hemostatic studies of fractions from raw and roasted sea buckthorn seeds in vitro

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dc.contributor.authorSławińska, Natalia
dc.contributor.authorŻuchowski, Jerzy
dc.contributor.authorStochmal, Anna
dc.contributor.authorOlas, Beata
dc.date.accessioned2024-09-19T13:39:20Z
dc.date.available2024-09-19T13:39:20Z
dc.date.issued2024
dc.description.abstractVarious seeds, including sea buckthorn (Hippophae rhamnoides L.) seeds, are sources of different bioactive compounds. They can show anti-inflammatory, hypoglycemic, anti-hyperlipidemic, antibacterial, antioxidant, or other biological properties in in vitro and in vivo models. Our preliminary in vitro results have demonstrated that the extracts from raw (no thermal processing) and roasted (thermally processed) sea buckthorn seeds have antioxidant potential and anticoagulant activity. However, it was unclear which compounds were responsible for these properties. Therefore, in continuation of our previous study, the extracts were fractionated by C18 chromatography. Phytochemical analysis of three fractions (a, b, and c) from raw sea buckthorn seeds and four fractions (d, e, f, and g) from roasted sea buckthorn seeds were performed. Several in vitro assays were also conducted to determine the antioxidant and procoagulant/anticoagulant potential of the fractions and two of their major constituents—isorhamnetin 3-O-β-glucoside7-O-α-rhamnoside and serotonin. LC–MS analyses showed that serotonin is the dominant constituent of fractions c and f, which was tentatively identified on the basis of its HRMS and UV spectra. Moreover, fractions c and f, as well as b and e, contained different B-type proanthocyanidins. Fractions b and e consisted mainly of numerous glycosides of kaempferol, quercetin, and isorhamnetin. The results of oxidative stress assays (measurements of protein carbonylation, lipid peroxidation, and thiol groups oxidation) showed that out of all the tested fractions, fraction g (isolated from roasted seeds and containing mainly dihexoses, and serotonin) demonstrated the strongest antioxidant properties.
dc.description.sponsorshipNarodowe Centrum Nauki, grant 2023/49/N/NZ9/00321
dc.identifier.citationSławińska, N., Żuchowski, J., Stochmal, A., Olas, B. Comparative phytochemical, antioxidant, and hemostatic studies of fractions from raw and roasted sea buckthorn seeds in vitro. Scientific Reports 2024, 14, 21175. https://doi.org/10.1038/s41598-024-72012-y
dc.identifier.doi10.1038/s41598-024-72012-y
dc.identifier.issn2045-2322
dc.identifier.urihttps://bc.iung.pl/handle/123456789/1848
dc.language.isoen
dc.publisherNature
dc.subjectOxidative stress
dc.subjectPlasma
dc.subjectSea buckthorn
dc.subjectSeeds
dc.subjectThermal processing
dc.titleComparative phytochemical, antioxidant, and hemostatic studies of fractions from raw and roasted sea buckthorn seeds in vitro
dc.typeArticle
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