Berberis vulgaris L. Root Extract as a Multi-Target Chemopreventive Agent against Colon Cancer Causing Apoptosis in Human Colon Adenocarcinoma Cell Lines

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dc.contributor.authorOch, Anna
dc.contributor.authorLemieszek, Marta Kinga
dc.contributor.authorCieśla, Marek
dc.contributor.authorJędrejek, Dariusz
dc.contributor.authorKozłowska, Aleksandra
dc.contributor.authorPawelec, Sylwia
dc.contributor.authorNowak, Renata
dc.date.accessioned2024-04-29T11:00:49Z
dc.date.available2024-04-29T11:00:49Z
dc.date.issued2024-04-27
dc.description.abstractBerberis vulgaris L. (Berberidaceae) is a shrub that has been widely used in European folk medicine as an anti-inflammatory and antimicrobial agent. The purpose of our study was to elucidate the mechanisms of the hemopreventive action of the plant’s methanolic root extract (BVR) against colon cancer cells. Studies were conducted in human colon adenocarcinoma cell lines (LS180 and HT-29) and control colon epithelial CCD841 CoN cells. According to the MTT assay, after 48 h of cell exposure, the IC50 values were as follows: 4.3, 46.1, and 50.2 g/mL for the LS180, HT-29, and CCD841 CoN cells, respectively, showing the greater sensitivity of the cancer cells to BVR. The Cell Death Detection ELISAPLUS kit demonstrated that BVR induced programmed cell death only against HT-29 cells. Nuclear double staining revealed the great proapoptotic BVR properties in HT-29 cells and subtle effect in LS180 cells. RT-qPCR with the relative quantification method showed significant changes in the expression of genes related to apoptosis in both the LS180 and HT-29 cells. The genes BCL2L1 (126.86–421.43%), BCL2L2 (240–286.02%), CASP3 (177.19–247.83%), and CASP9 (157.99–243.75%) had a significantly elevated expression, while BCL2 (25–52.03%) had a reduced expression compared to the untreated control. Furthermore, in a panel of antioxidant tests, BVR showed positive effects (63.93 0.01, 122.92 0.01, and 220.29 0.02 mg Trolox equivalents (TE)/g in the DPPH , ABTS +, and ORAC assays, respectively). In the lipoxygenase (LOX) inhibition test, BVR revealed 62.60 0.87% of enzyme inhibition. The chemical composition of BVR was determined using a UHPLC-UV-CAD-MS/MS analysis and confirmed the presence of several known alkaloids, including berberine, as well as other alkaloids and two derivatives of hydroxycinnamic acid (ferulic and sinapic acid hexosides). The results are very promising and encourage the use of BVR as a comprehensive chemopreventive agent (anti-inflammatory, antioxidant, and pro-apoptotic) in colorectal cancer, and were widely discussed alongside data from the literature.
dc.identifier.citationInternational Journal of Molecular Sciences, 2024, Vol. 25(9), 4786
dc.identifier.doi10.3390/ijms25094786
dc.identifier.issn1422-0067 (online) 1661-6596 (printed)
dc.identifier.urihttps://bc.iung.pl/handle/123456789/1222
dc.language.isoen
dc.publisherMDPI
dc.relation.ispartofseries2024, 25, 4786
dc.subjectphytopharmacology
dc.subjectBerberis vulgaris L.
dc.subjectantioxidants
dc.subjectanti-inflammatory
dc.subjectcolon cancer
dc.subjectLS180
dc.subjectHT-29
dc.subjectinflammatory bowel disease
dc.titleBerberis vulgaris L. Root Extract as a Multi-Target Chemopreventive Agent against Colon Cancer Causing Apoptosis in Human Colon Adenocarcinoma Cell Lines
dc.typeArticle
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