Signal molecules and enzymes produced by Rhizobium leguminosarum sv. trifolii strains originating from the subpolar and temperate climate zones as elements of adaptation to low temperature stress

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dc.contributor.authorJanczarek, Monika
dc.contributor.authorAdamczyk, Paulina
dc.contributor.authorGałązka, Anna
dc.contributor.authorMarzec-Grządziel, Anna
dc.contributor.authorWójcik, Magdalena
dc.contributor.authorPolakowski, Cezary
dc.contributor.authorMaciejczyk, Natalia
dc.contributor.authorBieganowski, Andrzej
dc.date.accessioned2025-06-02T07:07:05Z
dc.date.available2025-06-02T07:07:05Z
dc.date.issued2025
dc.description.abstractRhizobium leguminosarum sv. trifolii is a soil bacterium capable of establishing a nitrogen-fixing symbiosis with Trifolium spp. (clover) plants. In this study, two subpopulations of Rlt strains derived from the subpolar and temperate climate zones were characterized with respect to different physiological and metabolic traits. In addition, the influence of temperature on these processes was examined. Our results indicated that all the rhizobial strains grew at pH 5–9, tolerated salinity, and were sensitive to a majority of antibiotics. Moreover, the subpolar strains proved to be more tolerant to salinity than the temperate strains. A great majority of the strains (83.8 %) produced acyl-homoserine lactones in a wide range of temperatures (10–25 ◦C). Although no difference in the frequency of this trait between the subpopulations was found, more highly efficient AHL-producers were found among the temperate strains. In terms of the production of iron-chelating compounds and phosphate solubilization, half of the studied Rlt strains exhibited these traits, but more effective isolates were identified within the temperate subpopulation. These rhizobia also synthesized high amounts of indole-3-acetic acid and exhibited ACC deaminase activity, which is important for the regulation of the plant hormone ethylene. Some differences in phenotypic profiles between the individual strains were observed. The temperature range of 20–25 ◦C was optimal, whereas lower temperatures negatively affected the production of these molecules. In addition, the Rlt population exhibited a large diversity with respect to the auxiliary genetic content and metabolic potential. In conclusion, the Rlt strains produced a large set of signal molecules and enzymes and utilized a variety of carbon, nitrogen, phosphorus, and sulfur sources, which ensures their successful adaptation to various environments.
dc.description.sponsorshipThis study was financially supported by a grant from the National Science Centre (Poland) (no 2018/31/B/NZ9/00663).
dc.identifier.citationSoil Biology and Biochemistry 208 (2025) 109863
dc.identifier.doi10.1016/j.soilbio.2025.109863
dc.identifier.issn0038-0717
dc.identifier.urihttps://bc.iung.pl/handle/123456789/3272
dc.language.isoen
dc.publisherElsevier
dc.subjectACC deaminase
dc.subjectHomoserine lactones
dc.subjectIndole-acetic acid
dc.subjectLegumes
dc.subjectLow temperature stress
dc.subjectNitrogen-fixing symbiosis oxidase
dc.subjectSiderophores
dc.subjectRhizobia
dc.titleSignal molecules and enzymes produced by Rhizobium leguminosarum sv. trifolii strains originating from the subpolar and temperate climate zones as elements of adaptation to low temperature stress
dc.typeArticle
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