Anti-inflammatory and hemocompatibility of saponin fractions from wild-growing rupturewort (Herniaria L.) species: In vitro study and phytochemical analysis

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dc.contributor.authorKołodziejczyk-Czepas, Joanna
dc.contributor.authorPecio, Solomiia
dc.contributor.authorLiudvytska, Oleksandra
dc.contributor.authorPecio, Łukasz
dc.contributor.authorBandyszewska, Magdalena
dc.date.accessioned2025-11-27T12:03:11Z
dc.date.available2025-11-27T12:03:11Z
dc.date.issued2026-02-28
dc.description.abstractEthnopharmacological relevance: Members of the Herniaria (L.) genus are traditionally recommended to treat urinary tract diseases as well as other disorders, including inflammation of various etiology. Aim of the study: The aim of this study was to explore the biological activity of saponin fractions (1–50 μg/mL) derived from the whole herb of Herniaria glabra L. (HG) and H. polygama J. Gay (HP), and aerial parts of H. incana Lam (HI) in terms of their anti-inflammatory efficacy, influence on the blood coagulation cascade and fibrinolysis, and safety for blood cells. Materials and methods: Saponin fractions were characterized and quantified by UHPLC-HRMS spectrometer using in-house prepared herniariasaponin standards (HS1, HS4-HS19). Herniariasaponins (HS) were separated from the complex matrix using the combination of liquid-liquid partitioning, solid-phase extraction and gel filtration. Hemocompatibility of the fractions was examined in plasma, whole blood and isolated erythrocytes. Their anti-inflammatory efficacy and mechanisms of action were studied in the experimental systems models of peripheral blood mononuclear cells (PBMCs) and THP1-ASC-GFP reporter cells. Results: Bidesmosides of medicagenic and zanhic acids predominated (e.g., HS5, HS7) across all investigated saponin fractions, whereas HS1 and H10 were present only in the HG and HP fractions. The major monodesmosides of medicagenic acid denoted as HS6 and HS4 were detected in all studied species. Herniaria saponin fractions displayed considerable anti-inflammatory properties, primarily through modulation of the MYD88-IRAK1-IKK2/IKKβ signaling axis, resulting in suppression of NF-κB activation and inhibition of inflammasome formation. The fractions were also hemocompatible, with no effects on the coagulant and fibrinolytic properties of plasma. No toxic effects of the saponin fractions on isolated PBMCs or erythrocytes were detected at 1 and 5 μg/mL, and no hemolysis was found in whole blood across the concentration range of 1–50 μg/mL. Conclusions: Herniaria saponin fractions display good hemocompatibility and suppress inflammatory response at different molecular levels, including inhibitory effects on activation of signaling pathways and secretion of pro-inflammatory mediators.
dc.description.sponsorshipThis work was partly supported by a grant from the National Science Centre (2017/01/X/NZ1/00086) and (2018/30/M/NZ7/00111).
dc.identifier.citationJournal of Ethnopharmacology 2026; 357, 120953
dc.identifier.doi10.1016/j.jep.2025.120953
dc.identifier.issn0378-8741
dc.identifier.urihttps://bc.iung.pl/handle/123456789/4619
dc.language.isoen
dc.publisherElsevier
dc.subjectHerniaria L.
dc.subjectrupturewort
dc.subjectCaryophyllaceae
dc.subjectLC-MS
dc.subjecttriterpenoid saponins
dc.subjectcytotoxicity
dc.subjectanti-inflammatory
dc.subjectplasma
dc.subjecthemostasis
dc.subjectblood
dc.titleAnti-inflammatory and hemocompatibility of saponin fractions from wild-growing rupturewort (Herniaria L.) species: In vitro study and phytochemical analysis
dc.typeArticle
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