Effect of long-term radish (Raphanus sativus var. sativus) monoculture practice on physiological variability of microorganisms in cultivated soil

Simple item page
dc.contributor.authorNowak, Artur
dc.contributor.authorMajewska, Małgorzata
dc.contributor.authorMarzec-Grządziel, Anna
dc.contributor.authorOzimek, Ewa
dc.contributor.authorPrzybyś, Marcin
dc.contributor.authorSłomka, Anna
dc.contributor.authorKutyrieva-Nowak, Nataliia
dc.contributor.authorGałązka, Anna
dc.contributor.authorJaroszuk-Ściseł, Jolanta
dc.date.accessioned2024-07-29T15:38:18Z
dc.date.available2024-07-29T15:38:18Z
dc.date.issued2024
dc.description.abstractLong-term monoculture may affect soil environment biodiversity. An example of such a plant is radish (Raphanus sativus var. sativus), an economically important crop in Poland, a quick-growing vegetable with intensified harvest throughout the season. The aim of this study was to determine changes in biodiversity of soil under radish cultivation and to compare the research methods applied. The monoculture practice affected soil pH, but the organic carbon content remained stable. 16S RNA-seq analysis revealed changes in soil microbial population, with the dominant phyla Proteobacteria (37.3%), Acidobacteria (19%), and Actinobacteria (16%), and the dominant taxa Gaiella (1.59%), Devosia (1.51%) and Nocardioides (1.43%). These changes have not fully expressed in the number of culturable microorganisms, where only fungal abundance changed significantly. However, the physiological state of microbial cells (λ) indicated that oligotrophs and copiotrophs were in a vegetative (λ > 3.0) state at the beginning of the season and fungi at the end of the year. Changes in the biodiversity of soil microorganisms were visualised using Community Level Physiological Profiling, where an oscillation in Average Well Colour Development (OD560 = 0.78–1.48) was observed in successive months of radish culture, with biodiversity indices (Shannon and Substance richness) remaining similar. The greatest variation in the influence of monoculture practice on soil factors was observed for the soil enzymes activities (for dehydrogenase and peroxidase activities – 0.5 μg TPF/h/g DW and 1.5 μmolPYGL/h/g DW respectively). Alkaline phosphatases predominated among this group of enzymes, and the activity of carbon metabolism enzymes decreased over the season, except for invertases, where an increase in activity of up to 50 μg Glc/h/g DW was observed. All the parameters studied indicated changes in the soil environment. Nevertheless the microbial community remains stable during the whole experiment returning to equilibrium in a quite short time after changing conditions.
dc.description.sponsorshipResearch supported by the Ministry of Science and Higher Education of Poland as part of the statutory activities of the Department of Industrial and Environmental Microbiology, Maria Curie–Skłodowska University.
dc.identifier.citationJournal of Environmental Management Volume 367, September 2024, 122007
dc.identifier.doi10.1016/j.jenvman.2024.122007
dc.identifier.issn0301-4797
dc.identifier.urihttps://bc.iung.pl/handle/123456789/1826
dc.language.isoen
dc.publisherElsevier
dc.subjectmicrobiome variability
dc.subjectenzymes activity
dc.subjectmonocultures
dc.subjectbacterial community
dc.subjecteco-physiological diversity
dc.titleEffect of long-term radish (Raphanus sativus var. sativus) monoculture practice on physiological variability of microorganisms in cultivated soil
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Link do artykułu Effect of long-term radish (Raphanus sativus var. sativus) monoculture practice on physiological variability of microorganisms in cultivated soil.pdf
Size:
225.82 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
Artykuły