Browsing by Author "Wojciechowski, Kamil"

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    Surface activity of herniariasaponin and non-saponin fractions of Herniaria L. species
    (Elsevier, 2024-09-24) Pecio, Solomiia; Pecio, Łukasz; Wojciechowski, Kamil
    The study investigated the surface activity of aqueous extracts from three Herniaria L. species, namely H. glabra, H. polygama, and H. incana. The three crude extracts exhibited similar chromatographic saponin profiles and equilibrium surface tension values (46–47 mN/m at 1 % concentration). The adsorbed layers were predominantly elastic, with a high compression storage modulus, E'. However, they also showed the ability to dissipate mechanical energy over the aqueous-air interface, as evidenced by fairly high values of the surface compression loss modulus, E'' (45–50 mN/m). The extract of H. glabra exhibited a particularly high E' value (>150 mN/m). To analyze its complex chemical composition, the crude extract was fractionated using liquid chromatography. The sugar fraction was found to be dominant (∼65 % of the extract mass), but exhibited the lowest surface activity. As the acetonitrile content in the eluent increased, the subsequently eluted fractions showed an increasing ability to lower surface tension. The HGW30–70 fraction, which constitutes approximately 20 % of the crude extract mass, was the main contributor to the surface properties of the extract due to its herniariasaponins content. The HGW20–25-A fraction, which showed the second highest surface activity, was further separated into two sub-fractions: non-flavonoid and pure-flavonoid. The former components were primarily responsible for the surface activity and their composition resembled that of a more polar HGW10 fraction (terpene glycosides and C9-C12 fatty acids). The non-flavonoid subfraction was also responsible for solubilization of otherwise poorly water-soluble components, like flavonoids.