Browsing by Author "Trojak-Goluch, Anna"
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Item Aktualne i potencjalne możliwości uprawy tytoniu w warunkach występowania czarnej zgnilizny korzeni(Instytut Uprawy Nawożenia i Gleboznawstwa Państwowy Instytut Badawczy, 2008) Trojak-Goluch, AnnaItem Berkeleyomyces rouxiae - A Pathogen Causing the Black Root Rot of Tobacco(MDPI, 2024-12-18) Korbecka-Glinka, Grażyna; Trojak-Goluch, Anna; Czarnecka, DianaBlack root rot is a dangerous disease affecting many crops. It is caused by pathogens formerly known as Thielaviopsis basicola and then reclassified as two cryptic species, Berkeleyomyces basicola and B. rouxiae. The aim of this study was to perform species identification, morphological characterization, and pathogenicity tests for fungal isolates obtained from tobacco roots with black root rot symptoms in Poland. DNA sequences of the three regions (ITS, ACT, MCM7) were highly similar to the sequences of B. rouxiae deposited in the NCBI database. Phylogenetic analysis confirmed the assignment of the obtained isolates to this species. The cultures of four representative isolates (namely OT2, OT3, WPT7, WPT8) showed a similar structure and gray/brown color of the mycelium, although their growth rate varied from 3.8 to 5.1 mm/day depending on the isolate. The sizes of the endoconidia and chlamydospores showed a considerable variation, although they fit within ranges previously described for B. rouxiae. Pathogenicity tests performed on young tobacco plants grown in the inoculated peat substrate revealed differences among the four isolates. WPT7 demonstrated the lowest level of aggressiveness for tobacco. In contrast, the remaining three isolates caused severe disease symptoms and significantly reduced shoot and root dry weights of the susceptible cultivar Virginia Joyner. A parallel pathogenicity test performed on cultivar VRG 10TL confirmed the effectiveness of black root rot resistance derived from Nicotiana debneyi.Item Breeding of triploid common hop cultivars (Humulus lupulus L.)(Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy w Puławach) Trojak-Goluch, Anna; Skomra, UrszulaItem Kierunki badań naukowych wspierające doskonalenie genotypów chmielu(Instytut Uprawy Nawożenia i Gleboznawstwa Państwowy Instytut Badawczy, 2012) Skomra, Urszula; Przybyś, Marcin; Trojak-Goluch, AnnaItem Przydatność nowych kontaktowych i systemicznych środków chemicznych do usuwania pędów bocznych tytoniu(Instytut Uprawy Nawożenia i Gleboznawstwa Państwowy Instytut Badawczy, 2008) Trojak-Goluch, Anna; Berbeć, ApoloniuszItem SHOOT REGENERATION AND PLOIDY LEVEL OF PLANTS OBTAINED FROM STEM PITH TISSUE IN VITRO CULTURES OF TOBACCO (NICOTIANA TABACUM L.)(Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy w Puławach, 2020) Trojak-Goluch, AnnaItem The Use of Bacteria, Actinomycetes and Fungi in the Bioprotection of Solanaceous Crops against Tobacco Mosaic Virus (TMV)(MDPI, 2024) Trojak-Goluch, AnnaTobacco mosaic virus (TMV) is one of the most persistent and infectious plant viruses. The substantial economic losses caused by TMV in the production of tobacco and vegetables (especially in the Solanaceae family) are prompting the introduction of innovative solutions that effectively inhibit infection by this pathogen. Biological control agents based on bacteria of the genera Pseudomonas, Bacillus, Pantoea and actinomycetes are becoming increasingly popular in the fight against TMV. Some fungi, including Fusarium spp., Trichoderma spp., Alternaria spp. and Sepedonium spp., as well as wood-rotting fungi, also exhibit high anti-TMV activity. This article presents a comprehensive review of recent scientific advances in the bioprotection of selected solanaceous crops against TMV. It provides information on the structure of the virus, its host range, pathogenicity and the severity of losses caused in pepper, tomato and tobacco production. The review characterises environmentally safe techniques involving biological control agents naturally occurring in the environment and the bioactive compounds extracted from them. It also identifies their effects on crops at the morphological, physiological and molecular levels. In addition, the manuscript outlines prospects for the future applications of beneficial micro-organisms and active compounds derived from them in the protection against TMV.