Browsing by Author "Pecio, Solomiia"

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    Anti-inflammatory and hemocompatibility of saponin fractions from wild-growing rupturewort (Herniaria L.) species: In vitro study and phytochemical analysis
    (Elsevier, 2026-02-28) Kołodziejczyk-Czepas, Joanna; Pecio, Solomiia; Liudvytska, Oleksandra; Pecio, Łukasz; Bandyszewska, Magdalena
    Ethnopharmacological relevance: Members of the Herniaria (L.) genus are traditionally recommended to treat urinary tract diseases as well as other disorders, including inflammation of various etiology. Aim of the study: The aim of this study was to explore the biological activity of saponin fractions (1–50 μg/mL) derived from the whole herb of Herniaria glabra L. (HG) and H. polygama J. Gay (HP), and aerial parts of H. incana Lam (HI) in terms of their anti-inflammatory efficacy, influence on the blood coagulation cascade and fibrinolysis, and safety for blood cells. Materials and methods: Saponin fractions were characterized and quantified by UHPLC-HRMS spectrometer using in-house prepared herniariasaponin standards (HS1, HS4-HS19). Herniariasaponins (HS) were separated from the complex matrix using the combination of liquid-liquid partitioning, solid-phase extraction and gel filtration. Hemocompatibility of the fractions was examined in plasma, whole blood and isolated erythrocytes. Their anti-inflammatory efficacy and mechanisms of action were studied in the experimental systems models of peripheral blood mononuclear cells (PBMCs) and THP1-ASC-GFP reporter cells. Results: Bidesmosides of medicagenic and zanhic acids predominated (e.g., HS5, HS7) across all investigated saponin fractions, whereas HS1 and H10 were present only in the HG and HP fractions. The major monodesmosides of medicagenic acid denoted as HS6 and HS4 were detected in all studied species. Herniaria saponin fractions displayed considerable anti-inflammatory properties, primarily through modulation of the MYD88-IRAK1-IKK2/IKKβ signaling axis, resulting in suppression of NF-κB activation and inhibition of inflammasome formation. The fractions were also hemocompatible, with no effects on the coagulant and fibrinolytic properties of plasma. No toxic effects of the saponin fractions on isolated PBMCs or erythrocytes were detected at 1 and 5 μg/mL, and no hemolysis was found in whole blood across the concentration range of 1–50 μg/mL. Conclusions: Herniaria saponin fractions display good hemocompatibility and suppress inflammatory response at different molecular levels, including inhibitory effects on activation of signaling pathways and secretion of pro-inflammatory mediators.
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    Surface activity of herniariasaponin and non-saponin fractions of Herniaria L. species
    (Elsevier, 2024-09-24) Pecio, Solomiia; Pecio, Łukasz; Wojciechowski, Kamil
    The study investigated the surface activity of aqueous extracts from three Herniaria L. species, namely H. glabra, H. polygama, and H. incana. The three crude extracts exhibited similar chromatographic saponin profiles and equilibrium surface tension values (46–47 mN/m at 1 % concentration). The adsorbed layers were predominantly elastic, with a high compression storage modulus, E'. However, they also showed the ability to dissipate mechanical energy over the aqueous-air interface, as evidenced by fairly high values of the surface compression loss modulus, E'' (45–50 mN/m). The extract of H. glabra exhibited a particularly high E' value (>150 mN/m). To analyze its complex chemical composition, the crude extract was fractionated using liquid chromatography. The sugar fraction was found to be dominant (∼65 % of the extract mass), but exhibited the lowest surface activity. As the acetonitrile content in the eluent increased, the subsequently eluted fractions showed an increasing ability to lower surface tension. The HGW30–70 fraction, which constitutes approximately 20 % of the crude extract mass, was the main contributor to the surface properties of the extract due to its herniariasaponins content. The HGW20–25-A fraction, which showed the second highest surface activity, was further separated into two sub-fractions: non-flavonoid and pure-flavonoid. The former components were primarily responsible for the surface activity and their composition resembled that of a more polar HGW10 fraction (terpene glycosides and C9-C12 fatty acids). The non-flavonoid subfraction was also responsible for solubilization of otherwise poorly water-soluble components, like flavonoids.