Browsing by Author "Czubacka, Anna"

Now showing 1 - 9 of 9
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    DIVERSIFICATION OF DEFENSE RESPONSE TO POTATO VIRUS Y AMONG ALLOPLASMIC TOBACCO FORMS
    (Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy w Puławach) Czubacka, Anna; Depta, Anna; Doroszewska, Teresa
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    DIVERSIFICATION OF RESISTANCE RESPONSE OF SELECTED TOBACCO CULTIVARS (NICOTIANA TABACUM) DEPENDING ON THE USED POTATO VIRUS Y ISOLATES
    (Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy w Puławach, 2020) Depta, Anna; Doroszewska, Teresa; Czubacka, Anna
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    Improvement of salt tolerance in Vicia faba (L.) seedlings: a comprehensive investigation of the effects of exogenous calcium chloride
    (2025) Abo-Shanab, Walaa A. ; Elshobary, Mostafa E.; Czubacka, Anna; Diab, Rana H.
    Background This study investigated the effects of the different concentrations of CaCl2 (10 and 15 mol m−3) on the growth, physiology, and cytological characteristics of salt-stressed Vicia faba (L.) seedlings grown under greenhouse conditions. Results Salinity stress (150 mol m−3 NaCl) had detrimental effects on all measured growth parameters, increased the micronucleus count number (MCN) by 26.6 micronuclei/1000 cells, decreased the mitotic index (MI) by 66.6%, and caused various chromosomal aberrations, nuclear alterations, and chromatin bridges in salt-stressed seedlings compared to the untreated plant. Nevertheless, the seed priming with CaCl2 (10 and 15 mol m−3) significantly alleviated the toxic effects induced by salinity stress, improved growth parameters, total chlorophyll (TChl), proline, and total soluble sugar (TSS) contents in salt-stressed faba bean seedling compared with seedlings germinated from non-primed seeds. The antioxidative system of salt-stressed faba bean was highly stimulated by increasing the activity of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) enzymes as well as phenolics and flavonoids were increased in all salt stressed seedlings germinated from seeds primed with CaCl2 (10 and 15 mol m−3) indicating an improved tolerance of faba bean plant to salinity stress. Notably, the pretreatment with CaCl2 (10 mol m−3) reduced the micronuclei number per 1000 cells by 91.3% and decreased the abnormality index by 58.9% more effectively than CaCl2 (15 mol m−3). SDS-PAGE profiling revealed the presence of 16 proteins with different molecular weights, including two peptides, induced by CaCl2 (10 mol m−3) in response to salinity stress. Conclusions This study showed that 10 mol m−3 CaCl2 significantly improved salt tolerance in treated faba bean plants mitigating the antagonistic effects of salt stress on several physiological and cytological parameters.
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    Inheritance of Potato virus Y tolerance introgressed from Nicotiana africana to cultivated tobacco
    (Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy w Puławach) Korbecka-Glinka, Grażyna; Czubacka, Anna; Depta, Anna; Doroszewska, Teresa
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    Ocena odporności odmian i linii hodowlanych tytoniu na wirusa Y ziemniaka (PVY)
    (Instytut Uprawy Nawożenia i Gleboznawstwa Państwowy Instytut Badawczy, 2008) Doroszewska, Teresa; Czubacka, Anna
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    Possibilities of using Nicotiana species in breeding for virus resistance
    (Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy w Puławach, 2023) Depta, Anna; Doroszewska, Teresa; Czubacka, Anna
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    The Expression of Genes Involved in Synthesis of Bitter Acids and Xanthohumol and the Content of These Compounds in Aroma and Bitter Hop under Reduced Nitrogen Fertilisation
    (MDPI, 2024) Czubacka, Anna; Skomra, Urszula; Agacka-Mołdoch, Monika; Koziara-Ciupa, Marta
    Hop (Humulus lupulus) is cultivated for industrial purposes, primarily for brewing, but also for pharmaceutical and cosmetic applications. The hop cones are valued for their secondary metabolites, including bitter acids and prenylflavonoids. The objective of the presented study was to investigate the impact of reducing nitrogen fertilisation on the activity of genes involved in the production of bitter acids and xanthohumol in cultivars Lubelski (aroma) and Magnat (bitter) grown in the field. Furthermore, the content of these compounds was determined. The results demonstrated that the majority of studied genes were expressed at a similar level despite the reduction in the nitrogen dose by 25–30%. Nitrogen dose influenced the activity of genes BCAT2, HS1, and PAL. Moreover, the differences in gene expression were observed between cultivars and developmental stages. The reduction in nitrogen dose did not result in a reduction in secondary metabolite content. The content of alpha and beta acids in Magnat was approximately 14.8% and 3.2%, respectively, while in Lubelski it was approximately 7.7% and 5.8%, respectively. The content of xanthohumol in both cultivars was approximately 0.7%.
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    The Infection of Yellow Lupin (Lupinus luteus L.) with Bean Yellow Mosaic Virus (BYMV) and Cucumber Mosaic Virus (CMV) in Organic Farming in Eastern Poland
    (MDPI, 2025-09-25) Czubacka, Anna; Czarnecka, Diana ; Księżak, Jerzy
    Yellow lupin seeds are a rich source of protein, which is why they are grown for animal feed and human consumption. At the same time, there is growing interest in organic farming. However, this type of cultivation is more susceptible to diseases, including viral ones. Yellow lupin is most commonly affected by the bean yellow mosaic virus (BYMV) and cucumber mosaic virus (CMV). We have therefore determined the occurrence of these two pathogens in six new Polish yellow lupin cultivars (Goldeneye, Salut, Diament, Puma, Mister and Bursztyn) grown in accordance with organic farming rules. Field experiments were conducted over three years, from 2022 to 2024, in three locations in eastern Poland. The Goldeneye cultivar was the most susceptible to BYMV, with an average infection rate of 59.17% of plants. In contrast, the Puma cultivar was the least susceptible to BYMV infection, with an average infection rate of 23.34%. However, even within this cultivar, most plants were infected under conditions of strong pathogen pressure (up to 90% in one of the locations in 2024). CMV infections were less frequent, with no statistical differences being found between cultivars in terms of the number of infected plants.
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    Uzyskiwanie zdrowych sadzonek chmielu jako element restrukturyzacji odmianowej
    (Instytut Uprawy Nawożenia i Gleboznawstwa Państwowy Instytut Badawczy, 2008) Doroszewska, Teresa; Skomra, Urszula; Przybyś, Marcin; Czubacka, Anna; Grudzińska-Sterno, Magdalena