Browsing by Author "Bandyszewska, Magdalena"

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    Anti-inflammatory and hemocompatibility of saponin fractions from wild-growing rupturewort (Herniaria L.) species: In vitro study and phytochemical analysis
    (Elsevier, 2026-02-28) Kołodziejczyk-Czepas, Joanna; Pecio, Solomiia; Liudvytska, Oleksandra; Pecio, Łukasz; Bandyszewska, Magdalena
    Ethnopharmacological relevance: Members of the Herniaria (L.) genus are traditionally recommended to treat urinary tract diseases as well as other disorders, including inflammation of various etiology. Aim of the study: The aim of this study was to explore the biological activity of saponin fractions (1–50 μg/mL) derived from the whole herb of Herniaria glabra L. (HG) and H. polygama J. Gay (HP), and aerial parts of H. incana Lam (HI) in terms of their anti-inflammatory efficacy, influence on the blood coagulation cascade and fibrinolysis, and safety for blood cells. Materials and methods: Saponin fractions were characterized and quantified by UHPLC-HRMS spectrometer using in-house prepared herniariasaponin standards (HS1, HS4-HS19). Herniariasaponins (HS) were separated from the complex matrix using the combination of liquid-liquid partitioning, solid-phase extraction and gel filtration. Hemocompatibility of the fractions was examined in plasma, whole blood and isolated erythrocytes. Their anti-inflammatory efficacy and mechanisms of action were studied in the experimental systems models of peripheral blood mononuclear cells (PBMCs) and THP1-ASC-GFP reporter cells. Results: Bidesmosides of medicagenic and zanhic acids predominated (e.g., HS5, HS7) across all investigated saponin fractions, whereas HS1 and H10 were present only in the HG and HP fractions. The major monodesmosides of medicagenic acid denoted as HS6 and HS4 were detected in all studied species. Herniaria saponin fractions displayed considerable anti-inflammatory properties, primarily through modulation of the MYD88-IRAK1-IKK2/IKKβ signaling axis, resulting in suppression of NF-κB activation and inhibition of inflammasome formation. The fractions were also hemocompatible, with no effects on the coagulant and fibrinolytic properties of plasma. No toxic effects of the saponin fractions on isolated PBMCs or erythrocytes were detected at 1 and 5 μg/mL, and no hemolysis was found in whole blood across the concentration range of 1–50 μg/mL. Conclusions: Herniaria saponin fractions display good hemocompatibility and suppress inflammatory response at different molecular levels, including inhibitory effects on activation of signaling pathways and secretion of pro-inflammatory mediators.
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    Wound Healing Potential of the Salvianolic Acid H and Yunnaneic Acid B—The Rosmarinic Acid Derivatives: Anti-Inflammatory Action and Hemocompatibility In Vitro
    (MDPI, 2026-01-28) Liudvytska, Oleksandra; Krzyżanowska-Kowalczyk, Justyna; Kowalczyk, Mariusz; Bandyszewska, Magdalena; Skowrońska, Weronika; Bazylko, Agnieszka; Kolodziejczyk-Czepas, Joanna
    Phenolic acids of plant origin are recognized as key bioactive compounds with potential for both internal and topical applications. Although some of these phytochemicals are used for skin care and to improve wound healing, oligomeric derivatives of rosmarinic acid (RA) remain poorly characterized in this context. This study aimed to evaluate the anti-inflammatory potential of salvianolic acid H (SA H) and yunnaneic acid B (YA B) in experimental models related to wound-healing, specifically in skin cells (HaCaT keratinocyte and NHDF fibroblast lines), THP1-ASC-GFP monocytes, and human peripheral blood mononuclear cells (PBMCs). Both SA H and YA B reduced pro-inflammatory cytokine release from HaCaT, NHDF, and PBMCs with efficacy comparable to or exceeding that of RA. Analyses of intracellular pathways of inflammatory response revealed that SA H and YA B were also efficient inhibitors of inflammasome formation in THP1-ASC-GFP reporter cells. Furthermore, SA H showed significant inhibitory effects on the activities of cyclooxygenase-2 and 5-lipoxygenase (IC50 = 11.53 µg/mL and 2.41 µg/mL, respectively). None of the examined acids influenced the hemostatic system at concentrations of 1–5 μg/mL. At 50 μg/mL, a slight increase in plasma clotting rate was observed for SA H and RA. These findings indicate that SA H and YA B, two naturally occurring oligomeric derivatives of RA, exert significant anti-inflammatory activity and represent promising agents for further studies on their use to improve wound healing.